Bourne lab publications
Ruan S., Bourne, C.R. (2024) Escherichia coli Cells Evade Inducible ParE Toxin Expression by Reducing Plasmid Copy Number. Microbiol Spectr. 12(6):e0397323.
(click here)
(Review) Shengfeng Ruan, Chih-Han Tu, Christina R. Bourne (2024) Friend or Foe: Protein Inhibitors of DNA Gyrase. Biology 13(2): 84.
(click here)
Collaboration on ORPs with the Burgett group at OUHSC:
(this is an on-going active team project)
Severance, Z.C., Nuñez J.I., Le-McClain A.T., Malinky C.A., Bensen R.C., Fogle R.S., Manginelli G.W., Sakers S.H., Falcon E.C., Bui R.H., Snead K.J., Bourne C.R., Burgett A.W.G. (2023) Structure-Activity Relationships of Ligand Binding to Oxysterol-Binding Protein (OSBP) and OSBP-Related Protein 4. J. Med. Chem. 66:3866.
(click here)
Here is the culmination of many years work – and Kevin’s first author paper! We have found a weak spot in toxin-antitoxin interaction that may be amenable to manipulation
link to online version
Collaboration on DHFR crystallography with O’Reilly group:
Boyer, Z.W., Kessler, H., Brosman, H., Ruud, K.J., Falkowski, A.F., Viollet, C., Bourne, C.R., O’Reilly, M.C. (2022) Synthesis and Characterization of Functionalized Amino Dihydropyrimidines Toward the Analysis of their Antibacterial Structure-Activity Relationships and Mechanism of Action. ACS Omega 7:37907.
(click here)
Collaboration on crystallography with neighboring Singh group:
Johnson, B.P., Kumar, V., Scull, E.M., Thomas, L.M., Bourne, C.R., Singh, S. (2022) Molecular basis for the substrate promiscuity of isopentenyl phosphate kinase from Candidatus methanomethylophilus alvus. Chemical Reports 17:85.
(click here)
Snead, K.J. and Bourne, C.R. (2021) Intrinsic degradation of the Type-II antitoxin ParD from Pseudomonas aeruginosa. bioRxiv
(click here)
Kevin found that the ParD protein from this TA system was a pain in vitro because it kept degrading – so we decided to figure out parameters and resulting products for this phenomenon –
pretty interesting, check it out!
McGillick, J., Ames, J.R., Murphy, T., Bourne, C.R. (2021) A YoeB toxin cleave both RNA and DNA. Scientific Reports 11:3592 (doi, click here)
Christina R. Bourne (2021) Bacterial Growth Mindset: Structural Plasticity in Defense Systems. Structure 29:97 (doi, click here)
(sorry, not open access but contact me for a reprint!
(Review)
Chih-Han Tu, Michelle Holt, Shengfeng Ruan, Christina R. Bourne (2020) Evaluating the potential for cross-interactions of antitoxins in Type II TA systems. Toxins (Basel) 12(6): E422 (doi, click here)
Ames, J.R., McGillick, J., Murphy, T., Reddem, E., Bourne, C.R. (2020) Identifying a molecular mechanism that imparts species-specific toxicity to YoeB toxins. Frontiers in Microbiology 11:article 959
(doi, click here). PMCID: PMC7256200 PDB ID 6n90
Muddala, P.N., White, J.C., Nammalwar, B., Pratt, I., Thomas, L.M., Bunce, R.A., Berlin, K.D., Bourne, C.R. (2020) Inhibitor design to target a unique feature in the folate pocket of Staphylococcus aureus dihydrofolate reductase. European Journal of Medicinal Chemistry. (link)
PDB IDs: 6prd, 6prb, 6pra, 6pr6, 6pr7, 6pr8, 6pr9
Ames, JR., Muthuramalingam, M., Murphy, T., Najar, FZ., Bourne, CR. (2019) Expression of different ParE toxins results in conserved phenotypes with distinguishable classes of toxicity. Microbiology Open ePub 7/16/19 8:e902. (doi, click here). PMCID: PMC6813445
Muthuramalingam, M., White, J.C., Murphy, T., Ames, J.R., Bourne, C.R. (2018) The toxin from a ParDE toxin-antitoxin system found in Pseudomonas aeruginosa offers protection to cells challenged with anti-gryase antibiotics. Molecular Microbiology ePub 11/14/18 111(2): 441.
(doi, click here) PMCID: PMC6368863
(Review)
Muthuramalingam, M., White, J.C., Bourne, C.R. (2016) Toxin-Antitoxin Modules Are Pliable Switches Activated by Multiple Protease Pathways. Toxins (Basel) 8:e214
PMCID: PMC4963847
(Review)
Bourne, C.R. (2014) Utility of the biosynthetic folate pathway for targets in antimicrobial discovery. Antibiotics 3(1): 1-28. PMCID:PMC4790348
DHFR inhibitor discovery (research scientist projects, OSU)
Nammalwar, B., Bourne, C.R., Wakeham, N., Bourne, P.C., Barrow, E.W., Muddala, N.P, Bunce, R.A., Berlin, K.D., Barrow, W.W. (2015) Modified 2,4-diaminopyrimidine-based dihydrofolatereductase inhibitors as potential drug scaffolds against Bacillus anthracis. Bioorg. Med. Chem. 23:203-11. PMCID: PMC4278362
Kobayashi, M., Tomohiro, K., Koseki, Y., Bourne, C.R., Barrow, W., Aoki, S. (2014) Identification of novel potential antibiotics against Staphylococcus using structure-based drug screening targeting dihydrofolate reductase. J. of Chemical Information and Modelling, 28:1242-53. PMCID: PMC4334291
Nammalwar, B., Muddala, N.P., Bourne, C.R., Henry, M., Bourne, P.C., Bunce, R.A., Barrow, E.W., Berlin, K.D., Barrow, W.W. (2014) Synthesis and biological evaluation of 2,4-diaminopyrimidine-based antifolate drugs against Bacillus anthracis. Macromolecules 19:3231. PMCID: PMC4016962
Bourne, C.R., Wakeham, N., Webb, N. Nammalwar, B., Bunce, R.A., Berlin, K.D., Barrow, W.W. (2014) Structure and competitive substrate inhibition of dihydrofolate reductase from Enterococcus faecalis reveals restrictions to co-factor docking. Biochemistry 53:1228-1238. PMCID: PMC3985486
Bourne, C.R., Wakeham, N., Nammalwar, B., Tseitin, V., Bourne, P.C., Barrow, E.W., Ramnarayan, K., Bunce, R.A., Berlin, K.D., Barrow, W.W. (2013) Structure-activity relationship for enantiomers of potent inhibitors of B. anthracis dihydrofolate reductase. Biochimica et Biophysica Acta – Proteins and Proteomics epub Sept. 20, 2012. 1834:46-52. PMCID: PMC3530638
Nammalwar, B., Bunce, R. A., Berlin, K. D., Bourne, C. R., Bourne, P. C., Barrow, E. W., Barrow, W. W. (2013) Comparative Study of the Frech Catalyst with Two Conventional Catalysts in the Heck Synthesis of 2,4-Diaminopyrimidine-based Antibiotics. Organic Preparations and Procedures International. 45(1):66-71. PMCID: PMC3683996
Nammalwar, B., Bourne, C.R., Bunce, R.A., Wakeham, N., Bourne, P.C., Ramnarayan, K., Mylvaganam, S., Berlin, K.D., Barrow, E.W., Barrow, W.W. (2012) Inhibition of bacterial dihydrofolate reductase by 6-Alkyl-2,4-diaminopyrimidines. ChemMedChem epub Aug. 28. 7:1974-82. PMCID: PMC3570588
*Featured on inside cover figure
Nammalwar, B., Bunce, R.A., Berlin, K.D., Bourne, C.R., Bourne, P.C., Barrow, E.W., Barrow, W.W. (2012) Synthesis and biological activity of substituted 2,4-diaminopyrimidines that inhibit Bacillus anthracis. European Journal of Medicinal Chemistry 54:387-96. PMCID: PMC3408765
Nammalwar, B., Bunce, R. A., Berlin, K. D., Bourne, C. R., Bourne, P. C., Barrow, E. W., Barrow, W. W. (2012) Microwave-assisted Heck synthesis of substituted 2,4-diaminopyrimidine-based antibiotics. Organic Preparations and Procedures International. 44(3):281-87. PMC3691060
Nammalwar, B., Bunce, R.A., Berlin, K.D., Bourne, C.R., Bourne, P.C., Barrow, E.W., Barrow, W.W. (2012) Approaches to iodinated derivatives of vanillin and isovanillin. Organic Preparations and Procedures International. 44(2):146-152. PMCID: PMC3691065
Bourne, C.R., Barrow, E.W., Bunce, R.A., Bourne, P.C., Berlin, K.D., Barrow, W.W. (2010) Inhibition of antibiotic resistant Staphylococcus aureus by the broad-spectrum dihydrofolate reductase inhibitor RAB1. Antimicrobial Agents and Chemotherapy 54(9): 3825-33. PMCID: PMC2934973
Bourne, C. R., Bunce, R. A., Bourne, P. C., Berlin, K. D., Barrow, E. W., Barrow, W. W. (2009) Crystal structure of Bacillus anthracis dihydrofolate reductase with the dihydrophthalazine-based trimethoprim derivative RAB1 provides a structural explanation of potency and selectivity. Antimicrobial Agents and Chemotherapy 53(7): 3065-73. PMCID: PMC2704665
Publications with phenotypic screening (research scientist project, OSU):
Barrow, E.W., Clinkenbeard, P.A., Duncan-Decocq, R.A., Perteet, R.F., Hill, K.D., Bourne, P.C., Valderas, M.W., Bourne, C.R., Clarkson, N.L., Clinkenbeard, K.D., Barrow, W.W. (2012) High-throughput screening of a diversity collection using biodefense category A and B Priority pathogens. Journal of Biomolecular Screening 17(7):946-56. PMCID: PMC3700734
Bourne, C.R., Wakeham, N., Bunce, R.A., Nammalwar, B., Berlin, K.D., Barrow, W.W. (2012) Classifying compound mechanism of action for linking whole cell phenotypes to molecular targets. Journal of Molecular Recognition 25:216-23. PMCID: PMC3703735
To reproduce this calculation method, the following files are available: README_BiologCalcs Omnilog_output_example Proc_Stds_3conc_SUMdV_generic Database_SUMdVvalues Database_SUMdVvalues_culled
Publications in Hepatitis B virus structural virology and inhibition (post-doc research project, OUHSC):
Bourne, C. R., Katen, S. P., Fulz, M. R., Packianathan, C., Zlotnick, A. (2009) A mutant Hepatitis B virus core protein mimics inhibitors of icosahedral capsid self-assembly. Biochemistry, 48(8): 1736-42. PMCID: PMC2880625
Bourne, C., Lee, S., Venkataiah, B. Lee, A., Korba, B., Finn, M.G., Zlotnick, A. (2008) Small-molecule effectors of Hepatitis B virus capsid assembly give insight into virus lifecycle. Journal of Virology 82(20): 10262-70. PMCID: PMC2566253
Zlotnick, A., Lee, A., Bourne, C. R., Johnson, J. M., Domanico, P. L., Stray, S. J. (2007) In vitro screening for molecules that affect virus capsid assembly and other protein association reactions. Nature Protocols 2(3): 490-98. PMCID: PMC2099249
*Cover figure from this manuscript
Bourne, C. R., Finn, M. G., Zlotnick, A. (2006) Global structural changes in hepatitis B capsids induced by the assembly effector HAP1. Journal of Virology 80(22): 11055-61. PMCID: PMC1642186
Stray, S. J., Bourne, C. R., Punna, S., Lewis, W. G., Finn, M. G., Zlotnick, A. (2005). A heteroaryldihydropyrimidine activates and can misdirect Hepatitis B virus capsid assembly. Proceedings of the National Academies of Science USA, 102(23): 8138-40. PMCID: PMC1149411
Publications from earlier work and graduate studies (OMRF):
Ramsland, P. A., Terzyan, S. S., Cloud, G., Bourne, C. R., Farrugia, W., Tribbick, G., Geysen, H. M., Moomaw, C. R., Slaughter, C. A., Edmundson, A. B. (2006). Crystal structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody. Biochemical Journal, 395(3): 473-81. PMCID: PMC1462693
Terzyan, S. S., Bourne, C. R., Ramsland, P. A., Bourne, P. C., Edmundson, A. B. (2003). Comparison of the three-dimensional structures of a human Bence-Jones dimer crystallized on earth and aboard U.S. Space Shuttle Mission STS-95. Journal of Molecular Recognition, 16 (2): 83-90.
Bourne, P. C., Ramsland, P. A., Shan, L., Fan, Z.-C., DeWitt, C. R., Shultz, B. B., Terzyan, S. S., Moomaw, C. R., Slaughter, C. A., Guddat, L. W., and Edmundson, A. B. (2002). Three-dimensional structure of an immunoglobulin light-chain dimer with amyloidogenic properties. Acta Crystallographica, D58, 815-823.
Ramsland, P. A., Upshaw, J. L., Shultz, B. B., DeWitt, C. R., Chissoe, W. F., Raison, R. L., Edmundson, A. B. (2001). Interconversion of different crystal forms of Fabs from human IgM cryoglobulins. Journal of Crystal Growth, 232, 204-214.
Broyles, R. H., Belegu, V., DeWitt, C. R., Shah, S. N., Stewart, C. A., Pye, Q. N., Floyd, R. A. (2001). Specific repression of beta-globin promoter activity by nuclear ferritin. Proceedings of the National Academies of Science USA, 98(16), 9145-9150. PMC55387
Alvarado, U. R., DeWitt, C. R., Shultz, B. B., Ramsland, P. A., Edmundson, A. B. (2001). A method for growing protein crystals in capillary tubes. Journal of Crystal Growth, 233, 407-414.
Edmundson, A. B., DeWitt, C. R., Goldsteen, B. Z., Ramsland, P.A. (1999). Packing motifs as predictors of the propensity of antibody fragments to crystallize. Journal of Crystal Growth, 196, 276-284.
Edmundson, A. B., Goldsteen, B. Z., DeWitt, C. R., Fan, Z.-C., Shan, L., Faber, C., Hanson, B. L., Borrebaeck, C. A. K. (1998). Designing Fabs and Fvs with propensities to crystallize. The Immunologist, 6, 54-60.
